Plasmid_Backbone

Part:BBa_J179001:Design

Designed by: Jara Radeck   Group: Mascher Lab   (2013-12-20)

pBS2E: Bacillus subtilis vector, lacA integration, MLS resistance


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6237
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4581
    Illegal NotI site found at 6112
    Illegal NotI site found at 6243
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6237
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 6237
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 6237
    Plasmid lacks a suffix.
    Illegal XbaI site found at 6252
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1462
    Illegal SapI.rc site found at 2544


Design Notes

We removed any forbidden restriction sites by site-directed mutagenesis PCR. The multiple cloning site also includes a rfp for selection in E.coli.


Source

This part is an igem compatible version of the Bacillus subtilis vector pAX01

References

[http://www.ncbi.nlm.nih.gov/pubmed/11274134 Härtl et al.]